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| PN 5061A, B-Agonist ELISA Kit, Microtiter Plate (96T) |
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| DESCRIPTION |
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| Beta-Agonist, ELISA kit
PN 5061A, Microtiter Plate (96T)
This test is based on a mixture of antibodies raised against salbutamol and clenbuterol respectively. This "generic" test detects, due to the antibody mixture, more ß-agonists than the Clenbuterol-EIA. The test is recommended for the detection of the following ß-agonist (% cross-reactivity): salbutamol (100%), clenbuterol (100%), bromobuterol (100%), cimbuterol (75%), mapenterol (50%), mabuterol (60%), tulobuterol (50%), clenpenterol (50%), terbutaline (40%), carbuterol (40%), and cimaterol (10%). The dose-response curve shows a measuring range for salbutamol of 0.1-5 ng/ml or 5-250 pg/well. Urine can be used directly in this test after pH adjustment or to lower background after extraction. For faeces, cattle feed, tissue, plasma, bile, hair and choroid/retina samples, simple extraction methods are described in the users guide. The sample preparation procedures include an enzymatic hydrolysis to guarantee valid measurements of (glucorinated/sulphated) ß-agonists like salbutamol, terbutaline and carbuterol. Detection limits, depending on the ß-agonists, sample material and clean-up procedure, down to 0.05 ng/g can be reached. |
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