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| PN 5081C, Corticosteroids, ELISA Kit, Microtiter Plate (96T) |
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| DESCRIPTION |
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| Corticosteroids, ELISA kit
PN 5081C, Microtiter Plate (96T)
Synthetic corticosteroids are widely used in livestock breeding to treat inflammatory diseases. MRLs for Dexamethasone in milk, tissue/kidney and liver are 0.3, 0.5 and 2.5 ng/kg respectively. Corticosteroids are found in cattle feed and are suspected to be used as growth-promoters. In all European Community countries however, the use of growth promoters has been banned since 1986. The generic Corticosteroid-EIA is a competitive enzyme immunoassay for the screening of feed, milk and urine on the presence of the following Corticosteroids (% of cross-reactivity): Flumethasone (125%), Dexamethasone (100%), Betamethasone (93%), Triamcinolone (33%) and Prednisolone (21). The dose-response curve shows a measuring range for Dexamethasone of 0.125-4 ng/ml or 6-200 pg/well. Milk can be used directly in this test with a sensitivity down to 0.15 ng/ml. For cattle feed samples, extraction methods are described in the manual and sensitivities down to 20 ng/g can be obtained. Urine samples can be applied after a dilution in buffer or after an HPLC fractionation. Tissue samples need an three phase extraction and clean up procedure.
Product summary
User guide
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