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| PN 5101STZ, Sulfamethazine ELISA Kit, Microtiter Plate (96T) |
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| DESCRIPTION |
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| PN 5101SM, Microtiter Plate (96T)
The Sulfamethazine (Sulfadimidine) EIA is a competitive enzyme immunoassay for the screening of urine, tissue, plasma and milk samples on the presence of this antimicrobial. The dose-response curve shows a measuring range for sulfamethazine of 0.2 - 10 ng/ml. The antibody reacts with sulfamerazine (13% cross-reactivity) and shows high cross-reactivity (130%) towards the major metabolite N4-acetyl-sulfamethazine. The cross-reactivities of ten other sulfonamides (sulfadiazine, sulfadimethoxine, sulfadoxine, sulfaguanidine, sulfamethoxazole, sulfamethoxydiazine, sulfapyridine, sulfaquinoxaline, sulfathiazole and sulfatroxazole) are < 0.5%. Urine and plasma samples can be used to predict the level of sulfamethazine in tissues. In pig urine, acetylation is the major elimination pathway of sulfamethazine and about 75% is excreted as N4-acetyl-sulfamethazine. Due to the presence of the metabolite in urine and the sensitivity of the Sulfamethazine EIA, low concentrations in tissue can be screened for. Diluted urine, plasma and defatted milk samples can be analysed directly. For tissues, an extraction procedure is described. |
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